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massarray methylation profiling  (Sequenom)

 
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    Sequenom massarray methylation profiling
    Massarray Methylation Profiling, supplied by Sequenom, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/massarray+methylation+profiling/massarray+quantitative+methylation+analysis/pm38148658-188-75-74
    Average 90 stars, based on 1 article reviews
    massarray methylation profiling - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Methylation:

    Article Title: Gender dimorphism in hepatocarcinogenesis—DNA methylation modification regulated X‐chromosome inactivation escape molecule XIST
    Article Snippet: .. Methylation of CpG sites of the Xist first exon region in liver tissues between 10 WT and 10 Tet2 −/− female mice was quantified by Sequenom MassARRAY methylation profiling. ..

    Article Title: Gender dimorphism in hepatocarcinogenesis—DNA methylation modification regulated X‐chromosome inactivation escape molecule XIST
    Article Snippet: .. Methylation of XIST was detected by Sequenom MassARRAY methylation profiling between HCC tissues (T) and adjacent normal liver tissues (L). ..

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Expressing:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Western Blot:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Agarose Gel Electrophoresis:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..

    Methylated DNA Immunoprecipitation:

    Article Title: Gender dimorphism in hepatocarcinogenesis-DNA methylation modification regulated X-chromosome inactivation escape molecule XIST.
    Article Snippet: .. Scale bar, 50 μm. (G and H) The expression of YY1 in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by western blot analysis. (I) Expression of YY1 and XIST in HCC-1016 cells (knocked down YY1 in HCC-1016 cells by transfecting siRNA) by qPCR. (J) XIST was quantified using DNA agarose gel electrophoresis. (K) The 5-methylcytosine (5mC) level in the XIST promoter region by MeDIP assay and qPCR. was quantified by Sequenom MassARRAY methylation profiling. ..



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    <t>Monoallelic</t> <t>methylation</t> of the human DNMT1 gene. A , location of the DNMT1 CpG island/promoter <t>DNA</t> methylation assays used for bisulfite sequencing. Genomic coordinates on chromosome 19 are shown, along with location of individual CpG dinucleotides ( dashes ) and CpG island location ( gray bar ) in relation to the DNMT 1 gene (exon 1 shown as a blue box , intron shown as an arrowed line ) according to the University of California Santa Cruz genome browser (hg_18 assembly). The arrows denote transcriptional direction. The location of rs8112895 SNP in the DNMT1_assay_4 is also shown. B , representative DNA methylation of a single full term human placental sample for assay_1 ( i ) and assay_4 ( ii ) showing a monoallelic pattern of methylation within the human placenta. Between eight and 12 individual clones were sequenced for each sample. The circles correspond to CpG sites denoted by black dashes in A. Closed circles , methylation; open circles , lack of methylation. Mean methylation levels seen at each CpG site in multiple placental samples for each assay are shown as shaded bars between 0 and 100% ( n = 4). Missing circles indicate CpG sites for which no information was obtained. C , DNMT1_4 methylation assay spanning SNP rs8112895 was used for bisulfite sequencing methylation analysis in purified first trimester cytotrophoblast cells. i , reference genomic DNA sequence surrounding rs8112895 SNP ( R ) and CpG dinucleotides ( boxed ). ii , individual clone sequences showing the presence of bisulfite-converted CG sites ( TG ) and unconverted CG sites ( CG ) in both maternal and paternal alleles from this heterozygous sample. This confirms a lack of parent of origin of the observed DNMT1 promoter methylation.
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    Image Search Results


    Monoallelic methylation of the human DNMT1 gene. A , location of the DNMT1 CpG island/promoter DNA methylation assays used for bisulfite sequencing. Genomic coordinates on chromosome 19 are shown, along with location of individual CpG dinucleotides ( dashes ) and CpG island location ( gray bar ) in relation to the DNMT 1 gene (exon 1 shown as a blue box , intron shown as an arrowed line ) according to the University of California Santa Cruz genome browser (hg_18 assembly). The arrows denote transcriptional direction. The location of rs8112895 SNP in the DNMT1_assay_4 is also shown. B , representative DNA methylation of a single full term human placental sample for assay_1 ( i ) and assay_4 ( ii ) showing a monoallelic pattern of methylation within the human placenta. Between eight and 12 individual clones were sequenced for each sample. The circles correspond to CpG sites denoted by black dashes in A. Closed circles , methylation; open circles , lack of methylation. Mean methylation levels seen at each CpG site in multiple placental samples for each assay are shown as shaded bars between 0 and 100% ( n = 4). Missing circles indicate CpG sites for which no information was obtained. C , DNMT1_4 methylation assay spanning SNP rs8112895 was used for bisulfite sequencing methylation analysis in purified first trimester cytotrophoblast cells. i , reference genomic DNA sequence surrounding rs8112895 SNP ( R ) and CpG dinucleotides ( boxed ). ii , individual clone sequences showing the presence of bisulfite-converted CG sites ( TG ) and unconverted CG sites ( CG ) in both maternal and paternal alleles from this heterozygous sample. This confirms a lack of parent of origin of the observed DNMT1 promoter methylation.

    Journal: The Journal of Biological Chemistry

    Article Title: DNA Methylation-mediated Down-regulation of DNA Methyltransferase-1 ( DNMT1 ) Is Coincident with, but Not Essential for, Global Hypomethylation in Human Placenta

    doi: 10.1074/jbc.M109.064956

    Figure Lengend Snippet: Monoallelic methylation of the human DNMT1 gene. A , location of the DNMT1 CpG island/promoter DNA methylation assays used for bisulfite sequencing. Genomic coordinates on chromosome 19 are shown, along with location of individual CpG dinucleotides ( dashes ) and CpG island location ( gray bar ) in relation to the DNMT 1 gene (exon 1 shown as a blue box , intron shown as an arrowed line ) according to the University of California Santa Cruz genome browser (hg_18 assembly). The arrows denote transcriptional direction. The location of rs8112895 SNP in the DNMT1_assay_4 is also shown. B , representative DNA methylation of a single full term human placental sample for assay_1 ( i ) and assay_4 ( ii ) showing a monoallelic pattern of methylation within the human placenta. Between eight and 12 individual clones were sequenced for each sample. The circles correspond to CpG sites denoted by black dashes in A. Closed circles , methylation; open circles , lack of methylation. Mean methylation levels seen at each CpG site in multiple placental samples for each assay are shown as shaded bars between 0 and 100% ( n = 4). Missing circles indicate CpG sites for which no information was obtained. C , DNMT1_4 methylation assay spanning SNP rs8112895 was used for bisulfite sequencing methylation analysis in purified first trimester cytotrophoblast cells. i , reference genomic DNA sequence surrounding rs8112895 SNP ( R ) and CpG dinucleotides ( boxed ). ii , individual clone sequences showing the presence of bisulfite-converted CG sites ( TG ) and unconverted CG sites ( CG ) in both maternal and paternal alleles from this heterozygous sample. This confirms a lack of parent of origin of the observed DNMT1 promoter methylation.

    Article Snippet: Mass spectrometry-based DNA methylation profiling (SEQUENOM MassARRAY EPITYPER) revealed an absence of methylation of DNMT3A or -3B genes in all human tissues, including placenta and purified cytotrophoblasts ( supplemental Fig. 1 ).

    Techniques: Methylation, DNA Methylation Assay, Methylation Sequencing, Clone Assay, Purification, Sequencing

    DNMT1 promoter methylation is restricted to primates. Bisulfite DNA sequencing confirmed promoter methylation of the DNMT1 gene in full term baboon ( A ) and marmoset ( B ) placental tissue, with no evidence for methylation in mouse ( C ), bovine, or guinea pig placental tissue (data not shown).

    Journal: The Journal of Biological Chemistry

    Article Title: DNA Methylation-mediated Down-regulation of DNA Methyltransferase-1 ( DNMT1 ) Is Coincident with, but Not Essential for, Global Hypomethylation in Human Placenta

    doi: 10.1074/jbc.M109.064956

    Figure Lengend Snippet: DNMT1 promoter methylation is restricted to primates. Bisulfite DNA sequencing confirmed promoter methylation of the DNMT1 gene in full term baboon ( A ) and marmoset ( B ) placental tissue, with no evidence for methylation in mouse ( C ), bovine, or guinea pig placental tissue (data not shown).

    Article Snippet: Mass spectrometry-based DNA methylation profiling (SEQUENOM MassARRAY EPITYPER) revealed an absence of methylation of DNMT3A or -3B genes in all human tissues, including placenta and purified cytotrophoblasts ( supplemental Fig. 1 ).

    Techniques: Methylation, DNA Sequencing